Journal: Journal of Clinical Microbiology

Article Title: Comparison of the Vitek MS and Bruker Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems for Identification of Chryseobacterium Isolates from Clinical Specimens and Report of Uncommon Chryseobacterium Infections in Humans

doi: 10.1128/JCM.00712-18

Figure Lengend Snippet: Chryseobacterium identified at the species level by the Vitek MS and Bruker Biotyper systems compared to the results of 16S rRNA gene sequencing

Article Snippet: Among the 129 isolates identified at the species level, 78 and 39 isolates were identified as C. indologenes (type strain DSM 16777 T ; GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"LN681561","term_id":"732170053","term_text":"LN681561"}} LN681561 ) and C. gleum (type strain ATCC 35910 T ; GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"GL379781","term_id":"301087312","term_text":"GL379781"}} GL379781 ), respectively ( ).

Techniques: Sequencing

Journal: Journal of Clinical Microbiology

Article Title: Comparison of the Vitek MS and Bruker Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems for Identification of Chryseobacterium Isolates from Clinical Specimens and Report of Uncommon Chryseobacterium Infections in Humans

doi: 10.1128/JCM.00712-18

Figure Lengend Snippet: Chryseobacterium species misidentified by the Vitek MS and Bruker Biotyper systems compared to the results of 16S rRNA gene sequencing

Article Snippet: Among the 129 isolates identified at the species level, 78 and 39 isolates were identified as C. indologenes (type strain DSM 16777 T ; GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"LN681561","term_id":"732170053","term_text":"LN681561"}} LN681561 ) and C. gleum (type strain ATCC 35910 T ; GenBank accession no. {"type":"entrez-nucleotide","attrs":{"text":"GL379781","term_id":"301087312","term_text":"GL379781"}} GL379781 ), respectively ( ).

Techniques: Sequencing

Journal:

Article Title: Molecular Characterization of Yarrowia lipolytica and Candida zeylanoides Isolated from Poultry

doi:

Figure Lengend Snippet: Yeast species and strains examined and their sources

Article Snippet: Laboratory stock strains of Y. lipolytica and Candida species were also examined for comparison. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Species Strain(s) Source Candida zeylanoides Type, NRRL Y-6360 Georgia State University a 103, 130, 155 Smoked turkey drumstick b 157, 217 Turkey sausage b 121, 128 Roasted chicken breast b C. guilliermondii NRRL 2075 Georgia State University a C. krusei ( Issatchenkia orientalis ) NRRL 7179 Georgia State University a C. famata ( Debaryomyces hansenii ) CBS 767 Georgia State University a C. parapsilosis ATCC 22019 Georgia State University a C. sake CBS 159 Georgia State University a C. tropicalis CDC-59 Georgia State University a C. lipolytica 90-019095 Georgia State University a Yarrowia lipolytica 142, 160, 218 Chicken liver b 138, 253 Chicken liver II b 173, 175, 246 Roasted chicken breast b Open in a separate window a Courtesy of Sally Meyer. b From Ismail et al. ( 25 ).

Techniques:

Journal:

Article Title: Molecular Characterization of Yarrowia lipolytica and Candida zeylanoides Isolated from Poultry

doi:

Figure Lengend Snippet: (A) Direct ITS-PCR amplification from broth cultures and colonies. Lanes: 1, 3, and 5, Y. lipolytica strain 160; 2, 4, and 6, C. zeylanoides strain 155; 1 and 2: 36-h TGY broth culture; 3 and 4, 12-h TGY broth culture; 5 and 6, 36-h colony on TGY agar plate; M, DNA size marker. (B) Effect of cell concentration on amplification by direct ITS-PCR from 48-h TGY broth culture of Y. lipolytica strain 160. Cell populations (CFU per milliliter): lane 1, 3 × 108; lane 2, 3 × 106; lane 3, 3 × 104; lane 4, 3 × 103.

Article Snippet: Laboratory stock strains of Y. lipolytica and Candida species were also examined for comparison. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Species Strain(s) Source Candida zeylanoides Type, NRRL Y-6360 Georgia State University a 103, 130, 155 Smoked turkey drumstick b 157, 217 Turkey sausage b 121, 128 Roasted chicken breast b C. guilliermondii NRRL 2075 Georgia State University a C. krusei ( Issatchenkia orientalis ) NRRL 7179 Georgia State University a C. famata ( Debaryomyces hansenii ) CBS 767 Georgia State University a C. parapsilosis ATCC 22019 Georgia State University a C. sake CBS 159 Georgia State University a C. tropicalis CDC-59 Georgia State University a C. lipolytica 90-019095 Georgia State University a Yarrowia lipolytica 142, 160, 218 Chicken liver b 138, 253 Chicken liver II b 173, 175, 246 Roasted chicken breast b Open in a separate window a Courtesy of Sally Meyer. b From Ismail et al. ( 25 ).

Techniques: Amplification, Marker, Concentration Assay

Journal:

Article Title: Molecular Characterization of Yarrowia lipolytica and Candida zeylanoides Isolated from Poultry

doi:

Figure Lengend Snippet: Direct ITS-PCR amplification products and restriction fragments from Y. lipolytica and C. zeylanoides. Lanes 1 to 10, Y. lipolytica strains 160 (lanes 1 and 6), 138 (lanes 2 and 7), 173 (lanes 3 and 8), 246 (lanes 4 and 9), 175 (lanes 5 and 10); lanes 11 to 20, C. zeylanoides strains 155 (lanes 11 and 16), 130 (lanes 12 and 17), 103 (lanes 13 and 18), 121 (lanes 14 and 19), 128 (lanes 15 and 20). ITS-PCR amplicon, lanes 1 to 5 and 11 to 15; HinfI digest, lanes 6 to 10; HaeIII digest, lanes 16 to 20; M, DNA size marker.

Article Snippet: Laboratory stock strains of Y. lipolytica and Candida species were also examined for comparison. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Species Strain(s) Source Candida zeylanoides Type, NRRL Y-6360 Georgia State University a 103, 130, 155 Smoked turkey drumstick b 157, 217 Turkey sausage b 121, 128 Roasted chicken breast b C. guilliermondii NRRL 2075 Georgia State University a C. krusei ( Issatchenkia orientalis ) NRRL 7179 Georgia State University a C. famata ( Debaryomyces hansenii ) CBS 767 Georgia State University a C. parapsilosis ATCC 22019 Georgia State University a C. sake CBS 159 Georgia State University a C. tropicalis CDC-59 Georgia State University a C. lipolytica 90-019095 Georgia State University a Yarrowia lipolytica 142, 160, 218 Chicken liver b 138, 253 Chicken liver II b 173, 175, 246 Roasted chicken breast b Open in a separate window a Courtesy of Sally Meyer. b From Ismail et al. ( 25 ).

Techniques: Amplification, Marker

Journal:

Article Title: Molecular Characterization of Yarrowia lipolytica and Candida zeylanoides Isolated from Poultry

doi:

Figure Lengend Snippet: Cluster analysis of RAPD-ERIC2 patterns of Y. lipolytica and C. zeylanoides showing relations with the source of isolation. Dendogram was derived using UPGMA and Pearson product method. Reference strain, C. zeylanoidesT 6360. Other strains: C. zeylanoides 103, 158, 121, 128, 130, 155, and 217; Y. lipolytica 138, 142, 160, 173, 175, 218, 246, and 253; C. lipolytica 095.

Article Snippet: Laboratory stock strains of Y. lipolytica and Candida species were also examined for comparison. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Species Strain(s) Source Candida zeylanoides Type, NRRL Y-6360 Georgia State University a 103, 130, 155 Smoked turkey drumstick b 157, 217 Turkey sausage b 121, 128 Roasted chicken breast b C. guilliermondii NRRL 2075 Georgia State University a C. krusei ( Issatchenkia orientalis ) NRRL 7179 Georgia State University a C. famata ( Debaryomyces hansenii ) CBS 767 Georgia State University a C. parapsilosis ATCC 22019 Georgia State University a C. sake CBS 159 Georgia State University a C. tropicalis CDC-59 Georgia State University a C. lipolytica 90-019095 Georgia State University a Yarrowia lipolytica 142, 160, 218 Chicken liver b 138, 253 Chicken liver II b 173, 175, 246 Roasted chicken breast b Open in a separate window a Courtesy of Sally Meyer. b From Ismail et al. ( 25 ).

Techniques: Isolation, Derivative Assay

Journal:

Article Title: Molecular Characterization of Yarrowia lipolytica and Candida zeylanoides Isolated from Poultry

doi:

Figure Lengend Snippet: (A) Separation of chromosomal DNA from C. zeylanoides by CHEF PFGE. Lanes 1 and 9, C. zeylanoides type strain; lanes 2 and 8, isolates 217 and 158, respectively, from turkey sausage; lanes 3 and 5, isolates 128 and 121, respectively, from chicken breast; lanes 4, 6, and 7, isolates 130, 103, and 155, respectively, from smoked turkey; M, S. cerevisiae DNA size marker. Electrophoretic conditions: 100 V for 15 h with 120-s pulse time, followed by 180-s pulse time for 33 h at 14°C in 1% agarose gel. (B) Separation of chromosomal DNA from Y. lipolytica by CHEF PFGE. Lanes 1 and 4, isolates 218 and 160, respectively, from chicken liver 1; lanes 2, 3, and 6, isolates 246, 173, and 175, respectively, from chicken breast; lane 5, isolate 138 from chicken liver 2; M, S. cerevisiae DNA size marker. Electrophoretic conditions: 50 V for 36 h with 1,200-s pulse time, followed with 1,800-s pulse time for 36 h at 14°C in 0.75% agarose gel.

Article Snippet: Laboratory stock strains of Y. lipolytica and Candida species were also examined for comparison. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Species Strain(s) Source Candida zeylanoides Type, NRRL Y-6360 Georgia State University a 103, 130, 155 Smoked turkey drumstick b 157, 217 Turkey sausage b 121, 128 Roasted chicken breast b C. guilliermondii NRRL 2075 Georgia State University a C. krusei ( Issatchenkia orientalis ) NRRL 7179 Georgia State University a C. famata ( Debaryomyces hansenii ) CBS 767 Georgia State University a C. parapsilosis ATCC 22019 Georgia State University a C. sake CBS 159 Georgia State University a C. tropicalis CDC-59 Georgia State University a C. lipolytica 90-019095 Georgia State University a Yarrowia lipolytica 142, 160, 218 Chicken liver b 138, 253 Chicken liver II b 173, 175, 246 Roasted chicken breast b Open in a separate window a Courtesy of Sally Meyer. b From Ismail et al. ( 25 ).

Techniques: Marker, Agarose Gel Electrophoresis

Journal: Cornea

Article Title: Increased resistance of contact lens related bacterial biofilms to antimicrobial activity of soft contact lens care solutions

doi: 10.1097/ICO.0b013e3181a81835

Figure Lengend Snippet: List of strains tested in this study

Article Snippet: 32 table ft1 table-wrap mode="anchored" t5 caption a7 Species Isolate Source P. aeruginosa ATCC 9027 ATCC (from outer ear infection) * MRL8620 CMM * S. marcescens ATCC 13880 ATCC (from pond water) MRL9195 CMM 056SM Contact lenses of a patient with contact lens acute red eye (CLARE) in the Longitudinal Analysis of Silicone Hydrogel (LASH) contact lens study § 094SM Contact lenses of a patient with infiltrative keratitis the Longitudinal Analysis of Silicone Hydrogel (LASH) contact lens study § S. aureus ATCC 6538 ATCC (from a human lesion) ATCC 43300 ATCC (clinical isolate) 094SA Contact lenses of a patient with infiltrative keratitis the Longitudinal Analysis of Silicone Hydrogel (LASH) contact lens study § Open in a separate window * ATCC – American Type Culture Collection, Rockville, MD; CMM – Center for Medical Mycology (CMM), University Hospitals Case Medical Center.

Techniques: Infection

Journal: Cornea

Article Title: Increased resistance of contact lens related bacterial biofilms to antimicrobial activity of soft contact lens care solutions

doi: 10.1097/ICO.0b013e3181a81835

Figure Lengend Snippet: Growth kinetics of biofilms formed by P. aeruginosa, S. marcescens, and S. aureus on silicone hydrogel contact lens. Biofilm growth was monitored by determining the colony forming unit (CFU) count at each time point studied.

Article Snippet: 32 table ft1 table-wrap mode="anchored" t5 caption a7 Species Isolate Source P. aeruginosa ATCC 9027 ATCC (from outer ear infection) * MRL8620 CMM * S. marcescens ATCC 13880 ATCC (from pond water) MRL9195 CMM 056SM Contact lenses of a patient with contact lens acute red eye (CLARE) in the Longitudinal Analysis of Silicone Hydrogel (LASH) contact lens study § 094SM Contact lenses of a patient with infiltrative keratitis the Longitudinal Analysis of Silicone Hydrogel (LASH) contact lens study § S. aureus ATCC 6538 ATCC (from a human lesion) ATCC 43300 ATCC (clinical isolate) 094SA Contact lenses of a patient with infiltrative keratitis the Longitudinal Analysis of Silicone Hydrogel (LASH) contact lens study § Open in a separate window * ATCC – American Type Culture Collection, Rockville, MD; CMM – Center for Medical Mycology (CMM), University Hospitals Case Medical Center.

Techniques:

Journal: Cornea

Article Title: Increased resistance of contact lens related bacterial biofilms to antimicrobial activity of soft contact lens care solutions

doi: 10.1097/ICO.0b013e3181a81835

Figure Lengend Snippet: Comparison of the ability of clinical isolates and reference strains of P. aeruginosa, S. marcescens, and S. aureus to form biofilms on silicone hydrogel contact lenses, measured by determining the associated CFUs.

Article Snippet: 32 table ft1 table-wrap mode="anchored" t5 caption a7 Species Isolate Source P. aeruginosa ATCC 9027 ATCC (from outer ear infection) * MRL8620 CMM * S. marcescens ATCC 13880 ATCC (from pond water) MRL9195 CMM 056SM Contact lenses of a patient with contact lens acute red eye (CLARE) in the Longitudinal Analysis of Silicone Hydrogel (LASH) contact lens study § 094SM Contact lenses of a patient with infiltrative keratitis the Longitudinal Analysis of Silicone Hydrogel (LASH) contact lens study § S. aureus ATCC 6538 ATCC (from a human lesion) ATCC 43300 ATCC (clinical isolate) 094SA Contact lenses of a patient with infiltrative keratitis the Longitudinal Analysis of Silicone Hydrogel (LASH) contact lens study § Open in a separate window * ATCC – American Type Culture Collection, Rockville, MD; CMM – Center for Medical Mycology (CMM), University Hospitals Case Medical Center.

Techniques: Comparison

Journal: Cornea

Article Title: Increased resistance of contact lens related bacterial biofilms to antimicrobial activity of soft contact lens care solutions

doi: 10.1097/ICO.0b013e3181a81835

Figure Lengend Snippet: Gross morphology and surface topography of biofilms formed by P. aeruginosa, S. marcescens and S. aureus, compared with the corresponding planktonically grown cells. Scanning electron microscopy was performed to evaluate gross morphology and surface topography of biofilms formed by (A) P. aeruginosa, (C) S. marcescens and (E) S. aureus after 18h incubation. Panels B, D, and F represent planktonically grown cells of these three bacteria for 18 h, respectively. Arrow indicates biofilm matrix. Magnification, x5000.

Article Snippet: 32 table ft1 table-wrap mode="anchored" t5 caption a7 Species Isolate Source P. aeruginosa ATCC 9027 ATCC (from outer ear infection) * MRL8620 CMM * S. marcescens ATCC 13880 ATCC (from pond water) MRL9195 CMM 056SM Contact lenses of a patient with contact lens acute red eye (CLARE) in the Longitudinal Analysis of Silicone Hydrogel (LASH) contact lens study § 094SM Contact lenses of a patient with infiltrative keratitis the Longitudinal Analysis of Silicone Hydrogel (LASH) contact lens study § S. aureus ATCC 6538 ATCC (from a human lesion) ATCC 43300 ATCC (clinical isolate) 094SA Contact lenses of a patient with infiltrative keratitis the Longitudinal Analysis of Silicone Hydrogel (LASH) contact lens study § Open in a separate window * ATCC – American Type Culture Collection, Rockville, MD; CMM – Center for Medical Mycology (CMM), University Hospitals Case Medical Center.

Techniques: Electron Microscopy, Incubation, Bacteria

Journal: Cornea

Article Title: Increased resistance of contact lens related bacterial biofilms to antimicrobial activity of soft contact lens care solutions

doi: 10.1097/ICO.0b013e3181a81835

Figure Lengend Snippet: Confocal analysis of the architecture of biofilms formed by P. aeruginosa, S. marcescens and S. aureus. Panels show orthogonal view of biofilms formed on silicone hydrogel contact lens by (A) P. aeruginosa, (B) S. marcescens, or (C) S. aureus. Magnification, x40.

Article Snippet: 32 table ft1 table-wrap mode="anchored" t5 caption a7 Species Isolate Source P. aeruginosa ATCC 9027 ATCC (from outer ear infection) * MRL8620 CMM * S. marcescens ATCC 13880 ATCC (from pond water) MRL9195 CMM 056SM Contact lenses of a patient with contact lens acute red eye (CLARE) in the Longitudinal Analysis of Silicone Hydrogel (LASH) contact lens study § 094SM Contact lenses of a patient with infiltrative keratitis the Longitudinal Analysis of Silicone Hydrogel (LASH) contact lens study § S. aureus ATCC 6538 ATCC (from a human lesion) ATCC 43300 ATCC (clinical isolate) 094SA Contact lenses of a patient with infiltrative keratitis the Longitudinal Analysis of Silicone Hydrogel (LASH) contact lens study § Open in a separate window * ATCC – American Type Culture Collection, Rockville, MD; CMM – Center for Medical Mycology (CMM), University Hospitals Case Medical Center.

Techniques:

Journal: Cornea

Article Title: Increased resistance of contact lens related bacterial biofilms to antimicrobial activity of soft contact lens care solutions

doi: 10.1097/ICO.0b013e3181a81835

Figure Lengend Snippet: Activity of contact lens cleaning solutions against P. aeruginosa, S. marcescens, or S. aureus grown (A) as planktonic cells, or (B) as biofilms on lotrafilcon A lenses. The effect of contact lens care solutions against planktonically grown cells was evaluated according to International Organization for Standardization (ISO 14729) Stand Alone Procedure guidelines. The effect of contact lens care solutions against mature biofilm was assessed after soaking the biofilm coated lens in each manufacturer’s solution for the recommended soak time. Data represent reduction in log CFU (Mean ± Standard Deviation), for at least three replicates. *P ≤0.001 compared to untreated planktonic cells. Effects of Optifree (against P. aeruginosa and S. aureus) and Clear Care (against all three species) were significantly different (p<=0.02) from the biguanide preserved solutions. **P ≤ .001 when comparing effect of Clear Care solution against S. marcescens biofilms. ◆P ≤ .001 when comparing effect of OptiFree or Clear Care solutions against S. aureus biofilms.

Article Snippet: 32 table ft1 table-wrap mode="anchored" t5 caption a7 Species Isolate Source P. aeruginosa ATCC 9027 ATCC (from outer ear infection) * MRL8620 CMM * S. marcescens ATCC 13880 ATCC (from pond water) MRL9195 CMM 056SM Contact lenses of a patient with contact lens acute red eye (CLARE) in the Longitudinal Analysis of Silicone Hydrogel (LASH) contact lens study § 094SM Contact lenses of a patient with infiltrative keratitis the Longitudinal Analysis of Silicone Hydrogel (LASH) contact lens study § S. aureus ATCC 6538 ATCC (from a human lesion) ATCC 43300 ATCC (clinical isolate) 094SA Contact lenses of a patient with infiltrative keratitis the Longitudinal Analysis of Silicone Hydrogel (LASH) contact lens study § Open in a separate window * ATCC – American Type Culture Collection, Rockville, MD; CMM – Center for Medical Mycology (CMM), University Hospitals Case Medical Center.

Techniques: Activity Assay, Standard Deviation

Journal:

Article Title: Specific Detection of Pasteurella multocida in Chickens with Fowl Cholera and in Pig Lung Tissues Using Fluorescent rRNA In Situ Hybridization

doi: 10.1128/JCM.39.7.2627-2633.2001

Figure Lengend Snippet: Specificity test of oligonucleotide probe pmhyb449 and its complementary non-pmhyb449 probe labeled by Cy3 with bacteria grown in pure culture

Article Snippet: Twenty-two strains of P. multocida and other bacterial species isolated from normal and diseased lungs of poultry and other animals were obtained from publicly accessible service collections and a local culture collection (Table ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Bacterial strain a Bacterial species Animal source Hybridization signal intensity with b : pmhyb449 Non-pmhyb449 NCTC 10322 T P. multocida subsp. multocida Pig +++ − 214 P. multocida subsp. multocida Calf +++ − 40605-1 P. multocida subsp. multocida Eider +++ − RA 12/2 P. multocida subsp. multocida Calf +++ − NCTC 10204 T P. multocida subsp. gallicida Cow +++ − 77179 P. multocida subsp. gallicida Chicken +++ − NCTC 11619 T P. multocida subsp. septica Human c +++ − 5 P. avium biovar 2 Calf +++ − 25 P. canis biovar 2 Calf +++ − NCTC 11188 T P. gallinarum Chicken − − ATCC 43326 T P. canis biovar 1 Dog − − F 149 T P. anatis Duck − − CCM 5974 T Actinobacillus salpingitidis Chicken − − NCTC 4189 T A. lignieresii Cow − − P737 Mannheimia glucosida Sheep − − 14R525 E. coli Human − − SA 4461 Haemophilus paragallinarum Chicken − − SA 7191 H. paragallinarum Chicken − − 4237/2sv Riemerella anatipestifer Duck − − 4280/2sv R. anatipestifer Duck − − 726-82 T Coenonia anatina Duck − − 1912+pSD S. enterica serotype Gallinarum Chicken − − 19110+pSD S. enterica serotype Gallinarum Chicken − − Open in a separate window a A superscript T indicates the type strain. b +++, high signal intensity; −, no signal observed. c Infection was by means of a cat bite.

Techniques: Labeling, Hybridization

Journal:

Article Title: Specific Detection of Pasteurella multocida in Chickens with Fowl Cholera and in Pig Lung Tissues Using Fluorescent rRNA In Situ Hybridization

doi: 10.1128/JCM.39.7.2627-2633.2001

Figure Lengend Snippet: Oligonucleotide probe DNA sequence of the P. multocida -specific probe pmhyb449 compared with selected 16S rRNA sequences of strains of bacteria within the genera Pasteurella, Actinobacillus , and Mannheimia used for ISH

Article Snippet: Twenty-two strains of P. multocida and other bacterial species isolated from normal and diseased lungs of poultry and other animals were obtained from publicly accessible service collections and a local culture collection (Table ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Bacterial strain a Bacterial species Animal source Hybridization signal intensity with b : pmhyb449 Non-pmhyb449 NCTC 10322 T P. multocida subsp. multocida Pig +++ − 214 P. multocida subsp. multocida Calf +++ − 40605-1 P. multocida subsp. multocida Eider +++ − RA 12/2 P. multocida subsp. multocida Calf +++ − NCTC 10204 T P. multocida subsp. gallicida Cow +++ − 77179 P. multocida subsp. gallicida Chicken +++ − NCTC 11619 T P. multocida subsp. septica Human c +++ − 5 P. avium biovar 2 Calf +++ − 25 P. canis biovar 2 Calf +++ − NCTC 11188 T P. gallinarum Chicken − − ATCC 43326 T P. canis biovar 1 Dog − − F 149 T P. anatis Duck − − CCM 5974 T Actinobacillus salpingitidis Chicken − − NCTC 4189 T A. lignieresii Cow − − P737 Mannheimia glucosida Sheep − − 14R525 E. coli Human − − SA 4461 Haemophilus paragallinarum Chicken − − SA 7191 H. paragallinarum Chicken − − 4237/2sv Riemerella anatipestifer Duck − − 4280/2sv R. anatipestifer Duck − − 726-82 T Coenonia anatina Duck − − 1912+pSD S. enterica serotype Gallinarum Chicken − − 19110+pSD S. enterica serotype Gallinarum Chicken − − Open in a separate window a A superscript T indicates the type strain. b +++, high signal intensity; −, no signal observed. c Infection was by means of a cat bite.

Techniques: Sequencing

Journal:

Article Title: Specific Detection of Pasteurella multocida in Chickens with Fowl Cholera and in Pig Lung Tissues Using Fluorescent rRNA In Situ Hybridization

doi: 10.1128/JCM.39.7.2627-2633.2001

Figure Lengend Snippet: Photomicrographs demonstrating specific detection of P. multocida in pure culture and formalin-fixed lung tissues by use of the FISH technique. Shown are a pure culture of P. multocida subsp. multocida strain 40605-1 (A) and lung tissue sections of chicken (B, C, D, E, and F) or pig (G, H, and I) following light microscopy (C) or epifluorescence microscopy (A, B, and D through I) and ISH with the P. multocida-specific Cy3-labeled probe pmhyb449 (A, B, D, E, G, and H) and a nonsense Cy3-labeled probe, non-pmhyb449 (F and I), used as a control. A normal chicken lung (C) shows the location of the bacteria detected in cases of fowl cholera with an indication of the parabronchi (1), infundibulum (2), air capillary (3), pleura (4), and arterioles (5) (hematoxylin and eosin stain). Also shown are chicken lung infected with fowl cholera (experimentally infected with P. multocida subsp. multocida strain 40605-1), with the location of P. multocida cells near two blood vessels (artery and arteriole) (B), with P. multocida cells within the lumen and the walls of the air capillaries and perivascular space (D), and with P. multocida cells as individual organisms or as microcolonies (E); a control tissue section hybridized with probe non-pmhyb449 is also shown (F). Also shown is lung of a pig infected postmortem with P. multocida subsp. septica strain HIM 746-6T showing P. multocida bacteria in the lumen and wall of the alveoli (G), the area close to the pleura (H), and a control tissue section hybridized with non-pmhyb449 (I). Bars = 10 μm (A, B, D, E, F, G, H, and I) and 100 μm (C).

Article Snippet: Twenty-two strains of P. multocida and other bacterial species isolated from normal and diseased lungs of poultry and other animals were obtained from publicly accessible service collections and a local culture collection (Table ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Bacterial strain a Bacterial species Animal source Hybridization signal intensity with b : pmhyb449 Non-pmhyb449 NCTC 10322 T P. multocida subsp. multocida Pig +++ − 214 P. multocida subsp. multocida Calf +++ − 40605-1 P. multocida subsp. multocida Eider +++ − RA 12/2 P. multocida subsp. multocida Calf +++ − NCTC 10204 T P. multocida subsp. gallicida Cow +++ − 77179 P. multocida subsp. gallicida Chicken +++ − NCTC 11619 T P. multocida subsp. septica Human c +++ − 5 P. avium biovar 2 Calf +++ − 25 P. canis biovar 2 Calf +++ − NCTC 11188 T P. gallinarum Chicken − − ATCC 43326 T P. canis biovar 1 Dog − − F 149 T P. anatis Duck − − CCM 5974 T Actinobacillus salpingitidis Chicken − − NCTC 4189 T A. lignieresii Cow − − P737 Mannheimia glucosida Sheep − − 14R525 E. coli Human − − SA 4461 Haemophilus paragallinarum Chicken − − SA 7191 H. paragallinarum Chicken − − 4237/2sv Riemerella anatipestifer Duck − − 4280/2sv R. anatipestifer Duck − − 726-82 T Coenonia anatina Duck − − 1912+pSD S. enterica serotype Gallinarum Chicken − − 19110+pSD S. enterica serotype Gallinarum Chicken − − Open in a separate window a A superscript T indicates the type strain. b +++, high signal intensity; −, no signal observed. c Infection was by means of a cat bite.

Techniques: Light Microscopy, Epifluorescence Microscopy, Labeling, H&E Stain, Infection